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      Forensics

      Microreader™ 23sp

      Microreader™ 23sp

      Microreader™ 23sp ID System uses a 5-dye fluorescent technology and a co-amplification method to detect 23 loci including 21 non-CODIS loci, 1 CODIS loci, and Amelogenin. It is specifically designed for supplementary testing for paternity and kinship testing and other regular autosome detection. 


      For regular autosome kits for paternity and kinship analysis, please see "Microreader™ 21 ID System".

      Introduction

      The Microreader™ 23sp System is a 5-dye direct-amplification STR kit that amplifies 20 STR loci (including 13 CODIS loci) as well the highly discriminating Penta D and Penta E loci and a gender marker: Amelogenin. When individual locus of the case violates the laws of 1 sex chromosome: genetics, it can be used together with the normal CODIS kits to obtain a huge amount of information.

      The Kit is subjected to rigorous quality control testing to offer consistent performance. We develop and manufacture our products in accordance with ISO 9001 and ISO 18385 quality system requirements.


      Features

      ■ Allows direct amplification from these common sample types used in paternity testing without the need for extraction or purification, such as blood and buccal samples deposited on swab or paper substrates, as well as extracted DNA from various sample.
      ■ Superior discrimination ability, with cumulative non-father exclusion rate CPE>0.999 999 999.

      Superior discrimination performance

      As an addition to regular paternity and kinship testing, Microreader™ 23sp ID System greatly improves probability of discrimination power (DP) and excluding probability of paternity (PE) due to the choice of non-linked loci. The cumulative non-father exclusion rate CPE>0.999 999 999.

      Direct amplification & higher efficiency

      Without the need for sample extraction, purification and quantification, the entire process from direct amplification of the samples to the PCR product can be completed in 90 minutes, which improves the work efficiency and reduced testing costs, which are very suitable for the processing of large batches of samples. 

      High Compatibility

      As an autosomal complementary kit, it can be used together with the mainstream kits in the market, such as ABI: Globalfiler, Promega: VersaPlex 27PY, etc, from the angle of phylogenetic identification, increase the accuracy of screening.

      Kit related published paper

      [1] Wen, Dan, et al. "Genetic polymorphisms of new 22 autosomal STR loci in the Mongolian ethnic group." International journal of legal medicine 133.5 (2019): 1405-1407. (DOI: 10.1007/s00414-019-02111-3)
      [2] Liu, Qiu-Ling, et al. "Genetic polymorphism of 22 autosomal STR markers in a Han population of Southern China." Forensic Science International: Genetics 24 (2016): e14-e16. (DOI: 10.1016/j.fsigen.2016.06.017)

      Product Details

      Microreader™ 23sp Direct ID System Product Details

      For Use with (Application)

      Sequencing

      Unit size

      100/200 Reactions

      Label or Dye

      5 Dye

      Product type

      PCR amplification Kit

      Detection method

      Capillary Electrophoresis

      Required CODIS markers

      Covered

      Required ESS markers

      Covered

      Shipping Condition

      Dry ice

      Stroge Condition

      Store at –25 to –15°C upon receipt and at 2–8°C after initial use

      Target Loci

      D6S477, D18S535, D19S253, D15S659, D11S2368, D20S470, AMEL, D1S1656, D22-GATA198B05, D16S539, D7S3048, D8S1132, D4S2366, D21S1270, D13S325, D9S925, D3S3045, D14S608, D10S1435, D12S391, D2S1338, D17S1290, D5S2500